Fluorescence-activated Akt inhibitor cell sorter (FACS) analysis was applieded to determine the effects of resveratrol on cell apoptosis. Western blotting was performed to determine the protein levels of PKC alpha and ERK1/2. In inhibition experiments, HT-29
cells were treated with Go6976 or PD98059 for 30 min, followed by exposure to 200 mu M resveratrol for 72 h. Results: Resveratrol had a significant inhibitory effect on HT-29 cell growth. FACS revealed that resveratrol induced apoptosis. Western blotting showed that e phosphorylation of PKC alpha and ERK1/2 was significantly increased in response to resveratrol treatment. Pre-treatment with PKC alpha and ERK1/2 inhibitors (Go6976 and PD98059) promoted apoptosis. Conclusion: Resveratrol has significant anti-proliferative effects on the colon cancer cell line HT-29. The PKC-ERK1/2 signaling pathway can partially mediate resveratrol-induced apoptosis of HT-29 cells.”
“Background: Children with cerebral palsy (CP) have decreased strength, low bone mass, and an increased propensity to fracture. High-frequency, low-magnitude vibration might provide a noninvasive, nonpharmacologic, home-based treatment for these musculoskeletal deficits. The purpose of this study was to
examine the effects of this intervention on bone and muscle in children with CP.\n\nMethods: INK1197 Thirty-one children with CP ages 6 to 12 years (mean 9.4, SD 1.4) stood on a vibrating platform (30Hz, 0.3 g peak acceleration) at home for 10 min/d for 6 months and on the floor without the platform for another 6 months. The order of vibration and standing was randomized, and outcomes Buparlisib in vivo were measured at 0, 6, and 12 months. The outcome measures included computed tomography measurements of vertebral cancellous bone density (CBD) and cross-sectional area, CBD of the proximal tibia, geometric properties of the tibial diaphysis, and dynamometer measurements of plantarflexor
strength. They were assessed using mixed model linear regression and Pearson correlation.\n\nResults: The main difference between vibration and standing was that there was a greater increase in the cortical bone properties (cortical bone area and moments of inertia) during the vibration period (all P’s <= 0.03). There was no difference in cancellous bone or muscle between vibration and standing (all P’s > 0.10) and no correlation between compliance and outcome (all r’s < 0.27; all P’s > 0.15). The results did not depend on the order of treatment (P > 0.43) and were similar for children in gross motor function classification system (GMFCS) 1 to 2 and GMFCS 3 to 4.\n\nConclusions: The primary benefit of the vibration intervention in children with CP was to the cortical bone in the appendicular skeleton. Increased cortical bone area and the structural strength) properties could translate into a decreased risk of long bone fractures in some patients.