This survey contained questions regarding personal characteristics, running routines, and Selleckchem Enzalutamide previous RRI. Also a specific question was included to confirm that runners were injury-free before starting the follow-ups. All questions and details about the baseline survey are described in Appendix 1 (see eAddenda for Appendix 1) and were published elsewhere (Hespanhol Junior et al 2012). Data collection consisted of six follow-up surveys (Appendix 2, see eAddenda for Appendix 2) sent to the runners by email every 14 days throughout

the 12-week study period. Messages were sent by email every two weeks to remind the participants to complete the online survey for the previous fortnight. A reminder email was sent if the ON-01910 in vitro survey was not completed in three days. If runners had not completed the survey eight days after the initial email, they were then contacted by phone to remind them to complete the survey either online or over the phone. A reminder letter was sent by regular mail with a pre-paid return envelope if none

of the previous reminder attempts was successful. Participants who received a reminder by regular mail could complete a printed survey that had the same questions as the online version. In order to minimise the recall bias in the information collected in these follow-up surveys, we sent all runners a running log by regular mail to help them to record each running session. We requested that participants complete the running log with all relevant information and transfer these data while completing the fortnightly follow-up survey. The follow-up survey contained information about training, the presence of any RRI during the period, motivation to run, and any running races that the participant had competed in over the preceding two weeks. These questions elicited information about the following variables: number of times that the participant had trained; the total distance run (in kilometres); average time for each running session; predominant type of training surface (asphalt,

cement, grass, dirt, sand, gravel); Parvulin predominant type of terrain (flat course, uphill, downhill, or mixed); amount of speed training (ie, training sessions that include some bouts of high speed running during a very short period); number of interval training sessions as different running intensities (ie, Fartlek); motivation during training (motivated, neutral, or poorly motivated); amount and type of running races performed; and absence of training due to personal reasons, motivation, or unfavourable weather conditions (eg, rain). Participants were also asked whether they failed to train for at least one session due to the presence of any RRI during the period (see Question 12 in Appendix 2 on the eAddenda for details).