This finding proves that CK2-phosphorylation of eIF3j is a prerequisite for its association with the eIF3 complex. Expression of Ser127Ala-eIF3j mutant impairs both the interaction of mutated j-subunit with the other eIF3 subunits and the overall protein synthesis. Taken together our data demonstrate that CK2-phosphorylation of eIF3j at Ser127 promotes the assembly of the eIF3 complex, a crucial step in the activation of the translation initiation machinery. (C) 2015 Elsevier B.V. All rights reserved.”
“Hypoxia-inducible transcription factors HIF-1 alpha and HIF-2 alpha can contribute to pulmonary

hypertension and vascular remodeling, but their mechanisms remain unknown. This study investigated the role of HIF-1 alpha and HIF-2 alpha in pulmonary artery endothelial and smooth muscle cells. The exposure of human pulmonary artery endothelial cells (HPAECs) NU7441 in vitro to hypoxia (10% O-2 or 5% O-2) increased proliferation over 48 hours, compared with cells during normoxia (21% O-2). The adenovirus-mediated overexpression of HIF-2 alpha that is transcriptionally active during normoxia (mutHIF-2

alpha) increased HPAEC proliferation, whereas the overexpression of HIF-1 alpha, which is transcriptionally active during normoxia (mutHIF-1 alpha), exerted no effect. The knockdown of HIF-2 alpha decreased proliferation during both hypoxia and normoxia. Both HIFs increased migration toward fibrinogen, used as a chemoattractant. selleck In an angiogenesis tube formation assay, mutHIF-2 alpha-transduced cells demonstrated increased tube formation, compared with the mutHIF-1 alpha-transduced cells. In addition, the tubes formed in HIF-2 alpha-transduced cells were more enduring than those in the other groups. In human pulmonary artery smooth

muscle cells (HPASMCs), chronic exposure to hypoxia increased proliferation, check details compared with cells during normoxia. For HPASMCs transduced with adenoviral HIFs, HIF-1 alpha increased proliferation, whereas HIF-2 alpha exerted no such effect. Thus, HIF-1 alpha and HIF-2 alpha exert differential effects in isolated cells of the human pulmonary vasculature. This study demonstrates that HIF-2 alpha plays a predominant role in the endothelial growth pertinent to the remodeling process. In contrast, HIF-1 alpha appears to play a major role in pulmonary smooth muscle growth. The selective targeting of each HIF in specific target cells may more effectively counteract hypoxic pulmonary hypertension and vascular remodeling.”
“In Mainland China, many selection criteria for hepatocellular carcinoma (HCC) liver transplantation, such as the Hangzhou, the Chengdu, and the Fudan criteria, have been established.