The conserved site changes occurred in the absence of virological breakthrough at the following loci: rtR51K; rtL101F/L; and rtV173L, rtL180M, and rtM204V PD-0332991 cell line (Fig. 1A-C). Clonal analysis of the baseline sample from the lamivudine-naive patient with lamivudine resistance mutations demonstrated the presence of the rtV173L, rtL180M, and rtM204V mutational pattern at a frequency of 6.5% with

individual mutations present in up to 15% of the clones. Phenotypic analysis of the baseline and post-baseline isolates was performed for the three patients with post-baseline conserved site changes. Because the rtL101 change was observed as a mixture, a clone containing the full rtL101F change was also phenotyped to evaluate the impact of this substitution. The pHY92 laboratory strain and the

laboratory isolate containing the rtA181V and rtN236T ADV-associated mutations were used as controls for tenofovir sensitivity and reduced susceptibility, respectively. Overall, there was no change in tenofovir susceptibility within the three patients who developed conserved site changes in the pol/RT (Table 2). Among the 215 patients originally randomized to the ADV arms of the studies, 196 entered the OL-TDF phase. Nineteen of the 196 patients (9.7%) remained viremic after up to 96 weeks of OL-TDF; 1 discontinued TDF monotherapy at week 80, 14 patients added FTC to TDF between study weeks 72 and 120 (median time of TDF monotherapy = 30 weeks), 上海皓元 and 4 patients received 96 weeks of TDF monotherapy. The majority of the patients (11/19, 58%) showed no change in the pol/RT learn more versus the week 48 results (the last on-ADV results), 4 of 19 (21%) harbored polymorphic site changes, and 3 of 19 (16%) harbored distinct conserved site changes; PCR amplification failed for 1 patient (Table 1). The conserved site changes occurred at the following loci: rtG152E; rtA307A/T; and rtN236N/T and rtR274R/Q. Only

rtG152E was observed in the context of confirmed virological breakthrough (Fig. 1D-F). Phenotypic evaluations of a site-directed mutant containing the rtG152E substitution demonstrated that the virus remained susceptible to inhibition by tenofovir in vitro (Table 2), and the corresponding patient achieved undetectable HBV DNA levels with continued TDF monotherapy (Fig. 1D). Repeated attempts to obtain phenotypic results from either the patient pool or a clone containing the rtA307T substitution were unsuccessful, and the substitution was not observed upon subsequent genotypic testing. For patient 017, because the conserved site changes at rtN236 and rtR274 were observed as mixtures, individual clones containing the full changes were phenotyped. Phenotypic analysis of the viral pool remained sensitive to inhibition by tenofovir, as did a clone containing the single rtR274Q substitution.