Rhizoma Coptids alkaloids, which were quite possibly the most abundant constituents from the alcohol further of FTZ, exhibited a special fragmentation pathway during the optimistic ion mode. It is nicely identified that loss the neutral bcr-abl species which include CO, CH3, CH4 and CH2O have been observed within the MS2 spectra of Rhizoma Coptids alkaloids. Peak 23 showed a molecular ion at m/z 336 in MS spectra, and exhibited some ions at m/z 262 and 234 in MS2 spectra, displaying the neutral reduction of CO, CH3, CH4 and CH2O during the fragmentation pathway. These information are typical for your Rhizoma Coptids alkaloids in the present examine and steady with individuals within the literature. Thus, the compound was identied as berberine. Similarly, peaks 30 and 39 were identied as magnoorine, thalifendine, columbamine, fgfr4 inhibitor epiberberine, coptisine, jatrorrhizine, berberrubine, palmatine, dehydrocorydaline, 13 methylberberine and dehydrocorybulbine, respectively.

Peak 37 showed the molecular ion at m/z 398 and its product normal fragments at m/z 366 , 336 and 322 respectively from the MS2 spectrum. Based on the literature data, we advised that peak Lymph node 37 may very well be 13 ethyl dihydro 10 tetramethoxydibenzo quinolizinium. In addition to Rhizoma Coptids alkaloids in positive ion mode, 3 diterpenoids also exhibited ions in optimistic ion mode. It is effectively recognized that hydrogen at C 1 and oxygen at C 11 of tanshinones have been the source from the dissociated H2O and the neutral species like CO, H2O, C2H5 and C3H6 had been also observed in the MS2 spectra.

Peak 45 showed a molecular ion at m/z 297 in MS spectra, and exhibited an ion at m/z 279 in MS2 spectra, which corresponded to three fragment ions at m/z 268 , m/z 227 and m/z 251 , exhibiting the neutral reduction of CO, AKT Inhibitors H2O, C2H5 and C3H6 in the fragmentation pathway. In accordance with these information, peak 45 was tentatively identied as cryptotanshinone. Using precisely the same method, peak 43 and peak 49 have been identied as dihydrotanshinone I and tanshinone IIA by comparison with literature information and authentic standards. In addition, the molecular ion of peak 31 was observed during the MS spectra, which dissociated in MS2 to produce a number of ions at m/z 192 and 121. The ion at m/z 192 is often attributed to the reduction of the methyl radical from your mother or father ion, this ion fragmented even further with the reduction of CO to offer a signal at m/z 164. Subsequent reduction of a methyl in addition to a CO group radical to exhibited ions at m/z 149 and 121, had been observed. Evaluating with the genuine conventional and literature information, peak 31 was tentatively identied as 5, 7?dimethoxycoumarin. Peak 34 showed a molecular ion at m/z 343 in MS spectra, and exhibited 4 ions at m/z 295, m/z 181 , m/z 164 and m/z 120 in MS2 spectra, showing the loss of glucoside and hydroxy group inside the fragmentation pathway.