Range was established with five replicate readings of each concentration. Precision of the method was determined in the terms of intra-day and inter-day variation (%RSD). Intra-day precision (%RSD) was assessed by analysing standard drug solutions within the calibration range, three times on the same day. %RSD was found to be 0.30–1.14 for TDF and 0.51–1.37 for ETB. Inter-day precision (%RSD) was assessed by analysing drug solutions within the calibration range on three different days over a period of a week. Vismodegib concentration %RSD was found to be for TDF and 0.57–1.08 for ETB. This indicates that adequate preciseness of the method. Detection limit and quantification limit was calculated by the method as described in Section 2.4.2. The LOQ

and LOD for DAPT TDF were 13.99 ng and 42.40 ng. For ETB, LOQ and LOD were found to be 7.37 ng and 22.32 ng, respectively. This indicates that adequate sensitivity of the method. To the preanalysed sample a known amount of standard solution of pure drug (TDF and ETB) was over spotted at three different levels. These solutions were subjected to re-analysis by the proposed method and results of the same are shown in Table 2. The standard deviation of peak areas was calculated for each parameter and %R.S.D. was found 0.65–2.00. The low %R.S.D. indicates robustness of the method. The ruggedness of the proposed method was evaluated

by two different analysts. The results for TDF and ETB 3-mercaptopyruvate sulfurtransferase were found to be 99.78%, 99.50% and 100.64%, 100.28%, respectively. Repeatability of sample application was assessed by spotting (300 ng/spot) of drug solution seven times on a TLC, followed by development of plate and recording the peak area for seven spots. The %R.S.D. for peak

area values of TDF and ETB was found to be 1.21 and 0.57, respectively. The summery of validation parameters were listed in Table 3. The chromatogram of samples degraded with acid, base, hydrogen peroxide and light showed well separated spots of pure TDF and ETB as well as some additional peaks at different Rf values. The number of degradation product with their Rf values, content of TDF and ETB remained, and percentage recovery were calculated and listed in Table 4. The proposed HPTLC method provides simple, accurate and reproducible quantitative analysis for simultaneous determination of TDF and ETB in tablets. The method was validated as per ICH guidelines. All authors have none to declare. The authors are thankful to R.C. Patel College of Pharmacy for providing necessary facilities. “
“Diabetes associated complications have become a public health problem of considerable magnitude, because of huge premature morbidity and mortality associated with diabetes. Hyperglycemia inherent to diabetes patients accelerates accumulation of advanced glycation end-products (AGEs). Formation of AGEs is a slow non-enzymatic glycation process when reducing sugar reacts with proteins through a series of irreversible reaction and rearrangement.