PTS group translocators, like ABC transporters, are usually high affinity systems that recognize their sugar substrates with micromolar or sub-micromolar affinities. Since they use phosphoenolpyruvate to energize uptake, the same arguments presented for ABC transporters apply. Monocarboxylates (3.6% – 23 total) are transported by 15 secondary carriers and 11 primary active transporters. Di- & tricarboxylates and

aromatic compounds are transported solely by secondary carriers while noncarboxylic organoanions are mostly transported by secondary carriers. In summary, sugars are transported primarily by ATP-driven porters, while organic anionic compounds are transported primarily by pmf-driven carriers. This observation is in agreement with the primary energy source generated by the metabolism of these compounds (ATP from sugars; the pmf from organic acids). Amino acids & their derivatives are transported primarily by secondary carriers although peptides are taken Decitabine up almost exclusively by ABC systems. Transporters for amino acids and conjugates (9% – 56 total) include secondary carriers

(39 proteins), primary active transporters (16 proteins), and a single channel. Amines, amides, polyamines & organocations (2.4% – 15 total) were found to be transported by both primary active transporters (5 proteins) and secondary carriers (7 proteins). They are also transported by two amino sugar 5-Fluoracil order uptake group translocators (both TC# 4.A.1.1.5) and a channel protein (TC# 1.A.11.1.3). With the exception of one secondary carrier (TC# 2.A.17.1.1), almost all peptides (3.8% – 21 total) are taken up or expelled by primary active transporters (20 proteins). Considered collectively, nitrogenous compounds are thus transported roughly equally by primary and secondary carriers. Vitamins and especially iron siderophore complexes are primarily

taken up via ABC-type active transporters. Specifically, vitamins & vitamin or cofactor precursors are taken up by primary active transporters (5 proteins), secondary carriers (3 proteins) and a single group translocator. Transporters for siderophores and siderophore-Fe Thiamet G complexes (29 total) are mostly primary active transporters (21 proteins), with fewer secondary carriers (8 proteins). This fact probably reflects the need for high affinity recognition due to the low concentrations of these substances in the external environment. Transport of drugs and other hydrophobic substances occurs primarily by secondary pumps. Systems for multiple drugs (8.7% – 56 total) are exported via secondary carriers (36 proteins) and primary active transporters (20 proteins), but almost all of the specific drug exporters (62 total) are secondary carriers (58 proteins), with only four exceptional primary active transporters. By contrast, of the 8 pigment exporters identified [26, 27], 7 proved to be primary carriers. All other systems specific for hydrophobic substances are primary active transporters.