modiglianii from seven colonies, Their general abundance exceeded the hydro carbon abundance by the aspect five. 70 one. 39 SE, All novel compounds possessed eight equivalent diagnostic ions, which at most differed by 2 mass units, 3 even more ions were current during the majority of the 24 compounds, An preliminary comparison of their electron ionization mass spec tra with mass spectra from commercial libraries had proven high accordance with a fundamental steroid construction, In our prior review, we had consequently misinterpreted the mass spectra as indicative of the essential steroid structure. Nevertheless, the outcomes of HRMS too as mass spectra with the hydrogenated compounds will not help this original tentative interpretation, The molecular construction of compound 10 was more analysed. This compound occurred in all seven investigated colonies and, in five colonies, represented the single most abundant compound.
Primarily based on an extract of various tens of 1000′s of ants, we obtained 1H and 13C NMR information and ROESY and HMBC correlations, The construction elucidation revealed an octahydronaphthalene selleckchemWZ4003 subunit using a 2 butenoyl substituent likewise as an alkenyl moiety by using a terminal double bond, Consequently, the IUPAC name of compound ten is one two 1,two,4a,five,six,7,eight,8a octahydro naphthalene 1 yl but 2 en one 1, and we propose the trivial title crematoenone. The remaining 23 substances occurred on common in far lower abundances, which didn’t let NMR examination. However, based mostly on their equivalent mass spectra, we tenta tively presume that they are structurally related, Beside the name crematoenone for com pound 10 we for that reason propose to make use of the plural form crematoenones for your total substance class, reflecting the single double bond while in the octahydronaphtalene subunit and also the keto moiety.
For two additional compounds, we obtained ample substance to conduct HRMS evaluation, and primarily based around the reconstruction Trichostatin A 58880-19-6 and extrapolation of EI fragmentation mechanisms, we will propose sound chemical structures for these compounds, Compound 6 was tentatively identified as 2,3 dihydrocrematoenone. In compound 18, the double bond on the alkenyl moiety was in all probability replaced by an O acetyl performance, i. e. compound 18 will be an O acetyldihydrocrematoenone. Behavioural assays. Camponotus in direction of Crematogaster The behavioural assays have been to find out the role of hy drocarbons vs. crematoenones in nestmate recognition. We confronted a Camponotus rufifemur colony with dead Crematogaster modiglianii workers from its spouse colony and from a non nestmate Cr. modiglianii colony, and measured regardless of whether the Ca. rufifemur aggression differed be tween intracolonial and allocolonial Cr. modiglianii. In 3 subsequent treatment options, we similarly determined irrespective of whether Ca.