In this regard, it is interesting that, while vasodilatory influences generally predominate in pregnancy, the uterine circulation is unique in that myogenic tone increases late in pregnancy in the rat and in humans [58, 20] although, conversely, it decreases in guinea

pigs, mice, and sheep [42, 4, 24, 83, 85, 86]. A more detailed overview of the molecular mechanisms involved in gestational uterine vascular remodeling can be found in several recent reviews on the subject [59, 39, 73, 45]. Here, in view of space limitations, the authors would like to propose a mechanism that involves a series of temporally and spatially separated events that begin with a combination of increasing circulating and local concentrations of sex steroid hormones (estrogen, progesterone) and the process of placentation. Although the overall concept is hypothetical and not meant to be categorical, as species differences certainly exist, it does coalesce PI3K Inhibitor Library cell assay a number of established observations Hydroxychloroquine in vivo on the reported effects of sex steroids and growth factors, placentation, shear

stress, and endothelial signaling during pregnancy in different species, including the human. As already alluded to, increases in uterine artery diameter in humans begin well before placentation is complete, and expansive arterial remodeling can be initiated in rodents by inducing a pseudopregnant state in which increases in circulating sex steroids mimic those of pregnancy [82]. Estrogen in particular is a known vasodilator of the uterine circulation, and studies in the ewe [69] documented significant but transient increases in uterine blood flow in nonpregnant animals following a single injection of estradiol. A corollary to this observation is that the uterine circulation must normally possess a fair amount of intrinsic tone, as vasodilation can only be observed in a vessel that is already constricted. The mechanistic basis for this tone is not known, but may involve neural mechanisms because, of all regional circulations studied, the uterine is the most sensitive to the vasoconstrictor effects of catecholamines

such as norepinephrine [70]. Additional mechanisms, including endothelium-derived constricting factors and humoral influences, cannot be ruled out. The early expansive arterial remodeling is supplemented by the downstream process of hemochorial RG7420 cost placentation, which in rodents, guinea pigs, and primates (including humans), leads to the ablation of the endometrial microcirculation and the creation of a low velocity, high-flow chamber (the placenta). The key events involve both endovascular and perivascular trophoblast invasion of the maternal spiral arteries and placental development; a more detailed consideration of these processes can be found elsewhere [8, 21, 37]. The decrease in downstream resistance secondary to hemochorial placentation furthers an acceleration of the arterial blood in afferent maternal arteries, e.g.