At day P7, apoptosis and hypomyelination in periventricular white matter were evaluated by immunohistochemical assessments.
Results: High-dose maternal omega 3 FA treatment reduced brain weight loss. Maternal omega 3 FA treatment given either in low or high doses greatly decreased caspase-3 immunoreactivity and increased myelin basic protein immunoreactivity, indicating a decrease in apoptosis and hypomyelination. Conclusion: Considering that no specific Z-DEVD-FMK in vivo treatment is available for PVL, maternal omega 3 FA supplementation may provide a nutritional strategy to limit periventricular white matter damage caused by infections during pregnancy.”
“Introduction: Reversible protein cysteine oxidation is recognised as a pivotal post-transitional modification that transduces physiological
as well as pathological signalling. Pharmacological interventions that target specific sources of oxidant formation are currently being trialled learn more to ascertain their potential ability to prevent disease progression. To determine the selectivity of such pharmacological treatments and to indentify new drug targets, a suitable method is required to detect target cysteine oxidation. Method: Using a polyethylene glycol (PEG)-based alkylating agent the reversible oxidation of target proteins can be determined using a novel switch method. After reduction and specific labelling of reversibly oxidised thiols with a ‘heavy’ PEG-tag, samples are resolved on sodiumdodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, Western blotted and immunostained for protein(s) of interest. A mobility shift in a target protein following PEG-alkylation correlates with the reversible oxidative modification. Results: The oxidation
of cAMP-and cGMP-dependent protein kinases was detected using the PEG-switch assay Savolitinib datasheet in Langendorff-perfused hearts after hydrogen peroxide was administered. Discussion: The PEG-switch assay is a fast effective semi-quantitative method for measuring target reversible cysteine oxidation in complex protein mixtures derived from tissue or cultured cells. (C) 2013 Elsevier Inc. All rights reserved.”
“OBJECTIVES: Oncological surgery of the chest wall should be performed to achieve free margins of at least 2 cm for metastasis or 4 cm for primary tumours. When the lesion is not visible or palpable, difficulty in identification may lead to a larger incision and a resection wider than is necessary.
METHODS: We report three cases of non-palpable metastatic chest wall lesions in which the preoperative surgical planning and the intra-operative identification of the tumour, and thus the subsequent chest wall reconstruction, was supported using computer-based surgery.
RESULTS: The application of high-resolution three-dimensional imaging technology and navigational systems is used in preoperative surgical planning to provide virtual simulations of a patient’s skeletal changes and new soft tissue profile.