Acknowledgements We thank Alistair Graham for providing NSCLC sections, Stewart Church for assistance with phase-contrast microscopy and the Northern Ireland Leukaemia Research Fund for financial support. Electronic supplementary material Additional file 1: Loss of UCH-L1 expression did not Galunisertib mouse affect cell proliferation of H838 cells. CyQUANT® assays were performed at two different time points of 24 and 48 hr post-transfection with UCH-L1
siRNA in H838 cells. The results from 3 experiments are shown graphically. Statistical analysis showed no significant difference between UCH-L1 siRNA-treated and controls. (TIFF 444 KB) Additional file 2: Kaplan-Meier analysis in the GSE13213 dataset based on UCH-L1 expression. A. Kaplan-Meier analysis for patients separated into above and below the median of UCH-L1 expression in the GSE13213 dataset. B Kaplan-Meier analysis ATM inhibitor for patients separated into quartiles GSK461364 cell line based on UCH-L1 expression. The first and fourth quartiles are included in the graph. The UCH-L1 gene is represented by a single probe (A-23P132956). (TIFF 101 KB) Additional file 3: Kaplan-Meier analysis in the GSE3141 dataset based on UCH-L1 expression represented by probesets 1555834_at and 201387_s_at. A. Kaplan-Meier analysis for patients separated into above and below the median expression of UCH-L1 based on probeset 1555834_at signal intensities. B. Kaplan-Meier analysis for patients separated into quartiles based
on UCH-L1 expression represented by probeset 1555834_at. The first and fourth quartiles are included in the graph. C. Kaplan-Meier analysis for patients separated into above and below the median expression of UCH-L1 based on probeset Methane monooxygenase 201387_s_at signal intensities. D. Kaplan-Meier analysis for patients separated into quartiles based on UCH-L1 expression represented by 201387_s_at. The first and fourth quartiles are included in the graph. (TIFF 190 KB) Additional file 4: Kaplan-Meier analysis in the GSE8894 dataset based on UCHL-1 expression represented by 2 probesets 1555834_at and 201387_s_at. A. Kaplan-Meier analysis for patients separated into above
and below the median expression of UCH-L1 based on probeset 1555834_at signal intensities. B. Kaplan-Meier analysis for patients separated into quartiles based on UCH-L1 expression represented by probeset 1555834_at. The first and fourth quartiles are included in the graph. C. Kaplan-Meier analysis for patients separated into above and below the median expression of UCH-L1 based on probeset 201387_s_at signal intensities. D. Kaplan-Meier analysis for patients separated into quartiles based on UCH-L1 expression represented by 201387_s_at. The first and fourth quartiles are included in the graph. (TIFF 204 KB) References 1. Mani A, Gelmann EP: The ubiquitin-proteasome pathway and its role in cancer. J Clin Oncol 2005, 23:4776–4789.PubMedCrossRef 2. Mukhopadhyay D, Riezman H: Proteasome-independent functions of ubiquitin in endocytosis and signaling. Science 2007, 315:201–205.