The cypermethrin showed negative matrix effect for potato and water matrices, while deltamethrin, for these matrices, find more had a positive effect. This made the cypermethrin be located closest to the centre of the biplot graphic and deltamethrin closer to the tomato, pineapple and grape matrices. It is important to emphasise

that the behaviour of deltamethrin for the potato, water, apple, grape and tomato matrices was more significant at lower concentrations of the analyte. The iprodione and permethrin pesticides have shower negative effects for all matrices for tomatoes. The negative matrix effects presented were very significant, thus justifying the position in the biplot graphic in the same quadrant of tomato, pineapple and grape matrices when analysing the second component. According

to the results obtained in the PCA, it is clear that the matrices that caused an increase in the chromatographic response for most pesticides were tomato, pineapple and grape, which are acidic matrices. This suggests that pH is a variable that deserves to have its effects studied. Thus, to check the influence of pH on the matrix effect, all matrices studied had the pH determined. The values obtained were tomato (4.32), potato (5.74), water (6.65), apple (6.73), soil (6.76), pineapple (3.64), and grape (3.71). Water samples at pH 6.65 were adjusted to 4.32 (tomato pH), 3.64 SCH772984 order (pineapple pH), and 3.71 (grape pH) and submitted to LLE-PLT.

In addition, organic extracts of tomato, pineapple and grape were obtained by SLE-PLT as described in Table 1. Standard solutions of pesticides were prepared at a concentration of 500 μg L−1 in these six extracts and in pure solvent and analysed by GC-ECD. It was observed that water acidification promoted a reduction in pesticides chromatographic response similar to the results found for samples of pure water. This behaviour was also observed for the other pesticides studied. Thus, the pH of the samples does not influence selleck chemicals llc the properties of pesticides in the organic phase, and therefore the pH is not the directly responsible factor for the higher matrix effect observed for the more acidic samples. On the other hand, the increasing of the pH of the extracting mixture caused by the use of Na2HPO4 0.2 mol L−1 solution replacing the water used in SLE-PLT technique for samples of tomato, pineapple and grape, affected the extraction of the matrix components. Fig. 5 depicts the absorption spectra of organic extracts of the three matrices in two pH values. The spectra have the same characteristics, showing only that the organic extracts of these samples using pure water in the extracting mixture (