1   High 19.0 ± 1.0 20.4 ± 0.9 19.7 ± 1.2 Day 7 Control 23.0 ± 0.9 21.0 ± 1.2 22.0 ± 1.5   Low 22.6 ± 0.8 20.5 ± 1.0 21.6 ± 1.4   High 23.3 ± 1.2 20.7 ± 0.8 22.0 ± 1.7 Day 14 Control 27.0 ± 1.1 24.7 ± 0.8 25.8 ± 1.5   Low 27.1 ± 1.4 24.1 ± 1.2 25.6 ± 2.0   High 25.6 ± 1.3 22.7 ± 0.6* 24.2 ± 1.8 After treatment with Omipalisib carbon dots, the body weight of the mouse was weighed at different time points after the administration. Data were mean ± SD. *P < 0.05 compared with control by one-way ANOVA test. On the third ISRIB day after exposure, no significant difference was found among all groups in terms of the glutamic oxaloacetic transaminase (GOT), glutamate pyruvate transaminase

(GPT), urea, cholesterol, triacylglyceride (TG), blood glucose, total protein, and albumin levels (P > 0.05).

In contrast, the creatinine (Cr) levels in the high-dose group showed significant differences (P < 0.01), as shown in Table 2. Table 2 Biochemistry results of mice intravenously exposed to C-dots (day 3) Biochemical index Control (n = 10) Low (n = 10) High (n = 10) F value P value Glutamate-pyruvate transaminase (U/L) 40 ± 8 45 ± 15 43 ± 7 0.597 0.558 Glutamic oxaloacetic transaminase (U/L) 108 ± 22 111 ± 31 99 ± 15 0.697 0.507 Urea (mmol/L) selleck screening library 8.08 ± 1.79 6.79 ± 1.10 7.13 ± 2.08 1.521 0.237 Creatinine (μmol/L) 30 ± 2 28 ± 3 26 ± 2** 9.367 0.001 Cholesterol (mmol/L) 2.82 ± 0.25 2.68 ± 0.30 2.80 ± 0.50 0.428 0.656 Triglyceride (mmol/L) 1.39 ± 0.68 1.62 ± 0.56 1.44 ± 0.43 0.468 0.632 Blood glucose (mmol/L) 8.40 ± 1.38 8.17 ± 1.08 7.50 ± 0.80 1.749 0.193 Total protein (g/L) 52.8 ± 4.0 50.8 ± 2.6 51.0 ± 2.4 1.381 0.268 Albumin (g/L) 33.3 ± 3.0 32.0 ± 2.0 31.9 ± 2.2 1.147 0.333 The biochemical parameters of mice were determined 3 days after C-dot treatment. Data were mean ± SD. **P < 0.01 compared with that from mice in the control group by one-way ANOVA test. On the 14th day after exposure, no significant difference was found among all groups in their levels of GOT, GPT, urea, Cr, cholesterol, TG, PRKACG total protein, and albumin (P > 0.05). Blood glucose showed significant differences from the low-dose (P < 0.01) and high-dose (P < 0.05) groups compared with the control group (Table 3). The significant

decrease in the blood glucose concentration may be associated with the long duration of anesthesia. Table 3 Biochemistry results of mice intravenously exposed to C-dots (day 14) Biochemical index Control (n = 10) Low (n = 10) High (n = 10) F value P value Glutamate-pyruvate transaminase (U/L) 39 ± 11 41 ± 8 38 ± 8 0.352 0.707 Glutamic oxaloacetic transaminase (U/L) 104 ± 26 104 ± 20 94 ± 16 0.717 0.497 Urea (mmol/L) 7.66 ± 1.02 6.81 ± 1.25 6.87 ± 0.83 2.035 0.150 Creatinine (μmol/L) 24 ± 4 24 ± 3 23 ± 3 0.279 0.759 Cholesterol (mmol/L) 2.65 ± 0.50 2.67 ± 0.45 2.72 ± 0.48 0.050 0.951 Triglyceride (mmol/L) 1.66 ± 0.63 1.51 ± 0.29 1.66 ± 0.30 0.390 0.681 Blood glucose (mmol/L) 9.45 ± 1.33 7.76 ± 0.72** 8.34 ± 0.99* 6.795 0.004 Total protein (g/L) 52.2 ± 2.6 52.9 ± 2.0 52.4 ± 1.6 0.289 0.