Jeor equation [23] x an activity factor of 1.2. In an effort to decrease variability, the 500 kcal deficit was prescribed consistently to every subject based on estimated energy expenditures from the Mifflin-St. Jeor equation, as opposed to targeting the 500 kcal deficit to their baseline 3-day diet records. Each subject was given seven days of menus based off their daily allowance for calories. All menus consisted of three meals and two snacks and targeted a 40% carbohydrate, 30% protein and 30% fat eating plan. Each study participant was contacted on a weekly basis to assess compliance to diet and supplement

protocol. Subjects performed three, 60-minute exercise sessions per week using a ‘boot camp’ type of training. A typical class consisted of the following format: 10 minute warm-up (i.e. walking, light jogging, or biking); 30 minutes of circuit training learn more (upper and lower body each session) composed of the following exercises: mountain climbers, squat thrusts, jumping jacks, squat kickouts, walking lunges, push-ups, dips, resistance band elbow flexion, extension and shoulder presses; 10 minutes abdominals/core, and 10 minutes cool down/stretching. Based on pilot

data monitoring heart rate, this type of training expends approximately 300-400 kcal/session. Every training session was supervised SCH727965 molecular weight by a certified fitness Nepicastat in vitro professional and conducted at a single local facility to verify participation, and all subjects trained as one group. The fitness professional used a participant attendance log to monitor training compliance. All subjects had measurements of their weight, BMI, waist and hip girths, body fat and lean mass taken at week 0 (baseline), week 4 (midpoint of the study) and week 8 (end of mafosfamide the study). A member of the research staff contacted all subjects on a weekly basis to ensure compliance to the supplementation protocol, and pill counts were performed during mid and post testing. Blood samples were drawn at week 0 and week 8 for standard assessment of clinical laboratory parameters (i.e. comprehensive metabolic panel, lipid panel) and at weeks 0, 4 and 8 for serum concentrations

of adipocytokines (adiponectin, resistin, leptin, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)). Vital signs, including blood pressure and heart rate, were also recorded at weeks 0, 4 and 8. For each laboratory session, subjects reported to the laboratory normally hydrated (ad libitum water intake recorded prior to baseline testing and repeated prior to week 4 and week 8 testing), 12 hours postprandial and at least 48-hours following their last exercise session. All measurements were completed by the same researcher to minimize between-trial variation. Energy levels and food craving data were analyzed using a whole unit Likert-type scale [24]. Food craving was defined as “an intense desire for a specific food that is difficult to resist.