A random sample of older men and women stratified for age, sex, and expected 5-year mortality was drawn from the population registries of 11 municipalities in the Netherlands. The sampling and data collection procedures have been described in detail elsewhere [21, 22]. The sample for this study consisted of 1,509 participants

(65+ years) in the second cycle (1995/1996). In total, 1,427 participants had complete fall follow-up, of whom 1,342 participants had complete data (54 had missing values on physical activity and 31 on any of the confounders). Five additional participants were considered outliers and excluded from the analysis because of unlikely high values for physical activity. These five outliers all reported eight or more hours of light and heavy housekeeping activities per day, which is likely to Cilengitide mouse be due to over reporting. Moreover, their physical activity levels were more than four standard deviations away from the sample mean. In total, 1,337 participants were included in the analysis. The Medical Ethics Committee approved the study, and all participants signed informed consent. Falls and recurrent falling Falls were prospectively assessed during 3 years following the baseline

interview in 1995/1996 using a fall calendar [23]. Participants find more were asked to tick every week whether or not they had fallen. Once every 3 months, the calendar page was mailed to the institute. If the calendar procedure was too complicated, if the page was not received (even after a reminder), or if the page was completed incorrectly, the participants were contacted per telephone. Proxies were contacted if participants were unable to respond. A fall was defined as “an unintentional change in position resulting in coming to rest at a lower level or on the ground” [24]. Recurrent falling was defined as “falling

at least two times within 6 months during the 3-year fall follow-up” [25]. An occasional faller Dolutegravir concentration was defined as a person who fell at least once during follow-up, but who did not meet the criteria for recurrent falling. Time from baseline to the date of the first fall was Capmatinib nmr determined as time to first fall; time from baseline to the date of the second fall within a 6-month period was determined as the time to recurrent falling. Participants who were deceased, could not be contacted, or refused further participation during follow-up were included in the analyses until time of drop-out. Physical activity Physical activity was measured at baseline (1995/1996) using the validated LASA Physical Activity Questionnaire [26], an interviewer-administered questionnaire which estimates the frequency and duration of participation in activities in the previous 2 weeks. The activities were walking, cycling, light, and heavy household work and first and second sport.