2 %.Temperature CBL-0137 manufacturer of reaction: 60 °C for 18 h, mp: 172–174 °C (dec.). Analysis for C24H22N6O2S2 (490.60); calculated: C, 58.75; H, 4.52; N, 17.13; S, 13.07; found: C, 58.97; H, 4.51; N, 17.18; S, 13.10. IR (KBr), ν (cm−1): 3198 (NH), 3102 (CH aromatic), 2988, 1452, 759 (CH aliphatic), 1710 (C=O), 1605 (C=N), 1519 (C–N), 1329 (C=S), 693 (C–S). 1H NMR (DMSO-d 6) δ (ppm): 3.74 (s, 3H, CH3), 3.99 (s, 2H, CH2), 6.90 (d, J = 6 Hz, 2H, 2ArH), 7.32–7.56 (m, 10H, 10ArH), 7.57 (d, J = 6 Hz, 2H, 2ArH), 9.61, 9.66, 10.40 (3brs, 3H, 3NH). 4-Benzyl-1-[(4,5-diphenyl-4H-1,2,4-triazol-3-yl)sulfanyl]acetyl thiosemicarbazide (4h) Yield:

95.0 %. Temperature of reaction: 50 °C for 12 h, mp: 176–180 °C (dec.). Analysis for C24H22N6OS2 (474.60); calculated: C, 60.74; H, 4.67; https://www.selleckchem.com/products/GSK690693.html N, 17.71; S, 13.51; found: C, 60.77; H, 4.66; N, 17.78; S, 13.55. IR (KBr), ν (cm−1): 3209 (NH), 3087 (CH aromatic), 2971, 1439 (CH aliphatic), 1700 (C=O), 1611 (C=N), 1520 (C–N), 1351 (C=S), 689 (C–S). 1H NMR (DMSO-d 6) δ (ppm): 3.90 (s, 2H, CH2), 4.84 (s, 2H, CH2), 7.15–7.54 (m, 15H, 15ArH), 8.82, 9.54, 10.41 (3brs, 3H, 3NH). 13C NMR δ (ppm): 33.68 (–S–CH2–), 46.62 (–CH2–), 126.47, 127.12, 127.46, 127.83, 128.16, 128.51, 128.83, 129.83, 130.04 (15CH aromatic), 133.71, 134.71,

139.34 (3C aromatic), 151.95 (C–S), 154.32 (C-3 triazole), 166.79 (C=O), 182.09 (C=S). 4-(4-Methoxybenzyl)-1-[(4,5-diphenyl-4H-1,2,4-triazol-3-yl)sulfanyl]acetyl thiosemicarbazide (4i) Yield: 97.4 %. Temperature of reaction: 50 °C for 14 h, mp: 176–178 °C (dec.). Analysis for C25H24N6O2S2 (504.63); calculated: C, 59.50; H, 4.79; N, 16.65; S, 12.71; found: C, 59.61; H, 4.78; N, 16.68; S, 12.75. IR (KBr), ν (cm−1): 3222 (NH), 3102 CH (aromatic), 2973, 1448, 767 (CH aliphatic), 1697 (C=O), 1599 (C=N), 1514 (C–N), 1349 (C=S), 680 (C–S). 1H NMR (DMSO-d 6) δ (ppm): 3.76 (s, 3H, CH3), 4.01 (s, 2H, CH2), 4.74 (s, 2H, CH2), 6.86–7.64 (m, 14H, 14ArH), 8.33, 9.55, 10.44 (3brs, 3H, 3NH). 4-Ethoxycarbonyl-1-[(4,5-diphenyl-4H-1,2,4-triazol-3-yl)sulfanyl]acetyl

thiosemicarbazide (4j) Yield: 98.6 %. Temperature of reaction: 55 °C for 14 h, mp: 178–180 °C (dec.). D-malate dehydrogenase Analysis for C20H20N6O3S2 (456.54); calculated: C, 52.62; H, 4.41; N, 18.41; S, 14.05; found: C, 52.76; H, 4.42; N, 18.44; S, 14.01. IR (KBr), ν (cm−1): 3219 (NH), 3105 (CH aromatic), 2973, 1452, 765 (CH aliphatic), 1728 (C=O acidic), 1699 (C=O), 1608 (C=N), 1511 (C–N), 1338 (C=S), 691 (C–S).